Running the refinement

TODO: expliquer qu’on fait d’abord un petit raffinement C9 pour redresser le volume, puis un raffinement full data avec en initialisation le volume.

There are 2 ways to use the refinement protocol: 1. You have particles associated with poses. The protocol will output refined poses and an associated reconstruction. 2. You have particles but you don’t have their poses. The refinement will need a reference volume to initialize itself.

../../_images/ab-initio-aligned.png

The refinement will take as input the particles from the ab initio reconstruction output.

As the ab initio protocol, it will give you a reconstructed volume that you can visualise.

You’re done! Here is how your workflow should look like.

../../_images/scipion-tree.png