Tutorial

spfluo-app is a software aiming at bringing single particle reconstruction to fluorescence imaging.

This tutorial will guide you through the app. But first, install the app !

What’s covered here:

• Creating a project
• Importing images
• Visualise data with napari
• Picking particles manually
• Extracting particles
• Importing a PSF
• Running the ab initio reconstruction
• Alignment
• Gather more data with automatic picking
• Running the refinement
• Conducting experiments